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citG [2017-08-22 14:29:39]
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citG [2017-08-22 14:29:39]

fumarase
locus
BSU33040
pI
5.47
mw
50.36 kDa
protein length
462 aa Sequence Blast
gene length
1386 bp Sequence Blast
function
TCA cycle
product
fumarate hydratase
essential
no
ec
4.2.1.2
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]

    • Gene

    Coordinates
    3,389,024 → 3,390,412

    The protein

    Catalyzed reaction/ biological activity

  • (S)-malate = fumarate + H2O (according to Swiss-Prot)

    • Protein family

  • Fumarase subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|AnsB]

    • Structure

  • [PDB|1FUO] (aspartase from ''Bacillus'' sp. YM55-1, 64% identity) [Pubmed|21661762]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]
    description
    [Pubmed|3923430]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3130545,2509422,2509423], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3130545,2509422], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulation

  • constitutive
    • view in new tab

    genes
    [gene|B66AE23754A9CD4D5BCFC75CE6918522C7A22DA6|yvzF]-[gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • GP718 (spec), available in [SW|Jörg Stülke]'s lab [pubmed|20933603]
  • GP2340 (''[gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]''::''kan''), Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
  • GP2341 (''[gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]''::''lox72''), Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1122 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]

    • lacZ fusion

  • pGP387 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1433 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1132 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • **

    • References

  • 2509422,3923430,3130545,2509423,21661762,20933603,15378759,6098632